Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Staphylococcal entertotoxins of the enterotoxin gene cluster (egcSEs) induce nitric oxide- and cytokine dependent tumor cell apoptosis in a broad panel of human tumor cells

doi: 10.3389/fcimb.2013.00038

Figure Lengend Snippet: Anti- TNF-α and NO inhibitor, alone and in combination inhibit Hep-2 tumor cells cytotoxicity induced by 10% supernatants from SEA and egcSE-stimulated PBMCs . Hep-2 tumor cells cytotoxicity induced by 10% supernatants from SEA and egcSE-stimulated PBMCs before (black) or after treatment with L-NMMA (dark gray) or anti-TNF-α antibody (light gray) or a combination of L-NMMA plus anti-TNF-α antibody was analyzed by flow cytomerter using FITC-conjugated annexin-V and propidium iodide (IP) staining. The results are expressed as the mean ± SEM ( n = 3 independent experiments). Asterisks indicate statistical significance compared to values obtained before treatment with L-NMMA or anti-TNF-α antibody.

Article Snippet: PBMC supernatants were incubated with and without 30 μg/mL of mouse neutralizing polyclonal anti-TNF-α antibody (Abcys, Paris, France) for 18 h at 22°C before carrying out the cytotoxic assays described above.

Techniques: Staining

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Staphylococcal entertotoxins of the enterotoxin gene cluster (egcSEs) induce nitric oxide- and cytokine dependent tumor cell apoptosis in a broad panel of human tumor cells

doi: 10.3389/fcimb.2013.00038

Figure Lengend Snippet: Level of cytokines in supernatants of PBMC stimulated by SEA, SEG, SEI, SElM, SElN, and SElO .

Article Snippet: PBMC supernatants were incubated with and without 30 μg/mL of mouse neutralizing polyclonal anti-TNF-α antibody (Abcys, Paris, France) for 18 h at 22°C before carrying out the cytotoxic assays described above.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review

doi: 10.3390/ijms222312932

Figure Lengend Snippet: Animal studies included in the analysis. Abbreviations: 6-OHDA: 2,4,5-trihydroxyphenethylamine; DAI: disease activity index; DNBF: 2,4-dinitrofluorobenzene; DSS: dextran sulphate sodium; IA: iodoacetamide; MPO: mieloperoxidase; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; TNBS: 2,4,6-trinitrobenzene sulfonic acid; routes of administration: i.p.: intraperitoneal; i.g.: intragastric; i.v.: intravenous; s.c.: subcutaneous.

Article Snippet: Rooks et al., 2014 [ ] , TRUC colitis (T-bet−/−Rag2−/−) , Metronidazole (1 g/L, Sigma Aldrich, USA), gentamicin (2 g/L, Cellgro, USA), vancomycin (500 mg/L, Sigma Aldrich, USA), dissolved in drinking water; a hamster anti-mouse TNF-a neutralizing antibody (15 mg/kg, clone TN4-19.12, Bio X Cell, USA) given via i.v., FACS-sorted peripheral lymph node CD4+CD62LhiCD25+ cells (75 000 cells per mouse) given via i.v. , Homozygous T-bet−/−Rag2−/−(TRUC) SPF BALB/c mice and genobiotic TRUC BALB/c mice (Harvard School of Public Health, USA) , Up to 8 weeks , Histological scoring.

Techniques: Activity Assay, Generated, Ex Vivo, Retroviral, shRNA, Adjuvant, Concentration Assay, Knock-Out, Phagocytosis Assay